Tripsina-EDTA 1x (0,25% Tripsina/2,21 mM EDTA), 6 x 100 ml

Trypsin is available in several formulations, including solutions with or without EDTA. EDTA is a chelator that helps bind calcium and magnesium, allowing trypsin to access the cell-to-cell and cell-to-substrate bonds. More aggressive dissociation solutions contain higher concentrations of trypsin and EDTA, but higher concentrations can increase the risk of enzymatic damage to the cells. Higher concentration trypsin solutions should be used on a test monolayer prior to use.

When working in serum-free conditions, trypsin must be removed from contact with cells by centrifugation or deactivated by using a soybean trypsin inhibitor. Alternatively, use a non-enzymatic dissociation solution, such as Corning® Cellstripper® (25-056-CI), which gently dislodges loosely adherent cells using a mixture of chelators and does not require deactivation with serum or removal via centrifugation.