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Cultek, S.L.U.
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U.
Camí del Mig, 125 2a Planta, Puerta 35
08302 – MATARÓ (Barcelona)(Spain) Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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Anticuerpo IgG Rabbit anti-dog (cadenas pesadas y ligeras), conjugado con FITC (NORDIC-MUBIO)

Anticuerpo IgG Rabbit anti-dog (cadenas pesadas y ligeras), conjugado con FITC

  • 2 ml
  • -
  • Disponibilidad:
    • Cod. Prov: RAD_IGG(H_L)_FITC
    • -
    • Ref. Cultek: 03RAD/IGG(H+L)/FITC
    • -
    • Marca: NORDIC-MUBIO
Rabbit anti Dog IgG (heavy and light chains), conjugated with FITC
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Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

Background
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Source
Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure

Product
FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 2 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Product Form: FITC

Formulation: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Specificity
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Dog IgG, heavy and light chains.

Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine - Cat + Chicken - Duck - ferret + Goat - Guinea Pig + Hamster + Horse + Human + Mouse + Monkey + pigeon - Rat + Sheep - Swine + Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications
ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence.

Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

 

    • Referencia:
    • 03RAD/IGG(H+L)/FITC
    • Envase:
    • 2 ml
    • Categoría de Producto:
    • Anticuerpos secundarios
    • Subcategoría de Producto:
    • Secundarios
    • Temperatura Envío:
    • Ambiente
    • Temperatura Almacenaje:
    • Refrigerado 4º
    • Disponibilidad:
    • Disponible bajo pedido
    • Marca:
    • NORDIC-MUBIO
    • Aplicaciones:
    • Inmunoensayos
    • Especie/Huesped:
    • Conejo
    • Reactividad:
    • Perro
    • Isotipo:
    • IgG (heavy and light chains)
    • Conjugado:
    • FITC
Documentos