Contáctenos

Información de Contacto

Cultek, S.L.U.
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U.
Camí del Mig, 125 2a Planta, Puerta 35
08302 – MATARÓ (Barcelona)(Spain) Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

* Campos Requeridos

Para ver precios, disponibilidad y usar comparativas tiene que iniciar sesión o darse de alta

Transcriptasa reversa FastGene Scriptase II para RT-PCR, 20000 unidades, 100 reacciones (NIPPON GENETICS)

Transcriptasa reversa FastGene Scriptase II para RT-PCR, 20000 unidades, 100 reacciones

  • 100 rxns
  • -
  • Disponibilidad:
FastGene Scriptase II (20000 U)
Tiene que Iniciar sesión o Darse de alta para ver los precios de los productos

Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

Engineered enzyme

Engineered reverse transcriptases allowed the synthesis of cDNA from very low amounts of RNA. Mutations are inserted into the RNase H domain of the MuLV‘s reverse transcriptase. Therefore, by not degrading the RNA during the first-strand synthesis, a higher yield of full-length cDNA is obtained. Additionally, a higher thermal stability increases the robustness of the enzyme. The FastGene® Scriptase II is exactly one of those engineered enzymes. With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS.

Applications

  • Quantification of Gene Expression
  • RT-qPCR
  • Next Generation Sequencing
  • Low RNA concentration
  • Difficult templates

Lower RNase H activity for longer cDNA

The FastGene® Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12 kBp.

Engineered enzymes – optimized for qPCR

The FastGene® Scriptase II delivers superior cDNA templates for downstream applications, e.g. qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.

    • Referencia:
    • B4LS53
    • Envase:
    • 100 rxns
    • Categoría de Producto:
    • Retrotranscriptasas
    • Subcategoría de Producto:
    • MMLV
    • Temperatura Envío:
    • Congelado -20º
    • Temperatura Almacenaje:
    • Congelado -20º
    • Disponibilidad:
    • Disponible en 15 días laborables
    • Marca:
    • NIPPON GENETICS
    • Aplicaciones:
    • Amplificación (PCR y qPCR)
Documentos