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Información de Contacto

Cultek, S.L.U.  MADRID
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) 
Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U. BARCELONA
Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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Medio de congelación, Bambanker, 1 botella de 120ml (NIPPON GENETICS)

Medio de congelación, Bambanker, 1 botella de 120ml

  • 1 x 120 ml
  • -
  • Disponibilidad:
Bambanker (120 ml)
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Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

The freezing medium BambankerTM offered by the Japanese company Lymphotec was developed initially just for their own R&D projects. They needed a suitable medium for long-term storage of highly sensitive cell lines, such as lymphocytes.

Thanks to the innovative formulation of the new freezing medium, an European Patent (EP 1347040) was granted and the development of this media was made commercially available. Today this innovative cell freezing medium BambankerTMis the market leader in Japan and characterized by many different published articles with very sensitive cell lines all over the world.

Universal – even for preservation of stem cells

All common cell lines can be preserved resulting in a high number of intact cells after thawing. Recovery rates of  even sensitive cells is much higher compared to regular media.

User friendly with a high stability

No dilution, no addition of components (like DMSO or glycerol). No programmable freezer and no sequential freezing steps are required – saves a lot of time. Shelf life is 24 months, when stored in a fridge (2 – 10 °C).

Innovative

Because of the unique formulation, Lymphotec Inc. has got a European patent (EP 1347040).

Flexible

1 x 20, 5 x 20 and 1 x 120 ml bottles.

    • Referencia:
    • B4BB01
    • Especificaciones:
    • Cultivation  

      15% FBS/DMEM (1 mM of Sodium pyruvate, 100 µM of NEAA, 100 µM of ß-ME, 1000 U/ml of LIF) was used as culture medium. Mouse Embryonic Fibroblasts (MEF) were used as „feeder cells“.

      Freezing

      Cells were frozen in 5 vials / ( 60mm dish corresponds to 3.0 x 106 cells/vial ). 1 ml/vial of BambankerTM freezing medium was added and the mixture was directly frozen in -80 °C. The following day, the vials were transferred to liquid nitrogen (slow freezing).

      Thawing

      Cells were incubated at 37 °C and transferred in cooled culture media. After collection, cells were seeded in 6 well plates and 6 cm dishes.

      Results

      Stabilization of Mouse Embryonic Stem Cells by using BambankerTM was succesful. Cells were undifferentiated, even after freeze and thaw procedure. No modifications of cells could be observed.
      Data were kindly provided by Dr. Ahn (Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Department of Biomolecular Engineering, Tagawa Laboratory, Japan).
      Die Daten wurden von Dr. Ahn (Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Department of Biomolecular Engineering, Tagawa Laboratory, Japan ) zur Verfügung gestellt.
    • Caracteristicas:
      • Enables long term cell storage at -80°C or -196°C
      • Improved cell recovery and viability after thawing
      • Ready-to-use freezing media – no programmed or sequential freezing required
      • Serum-free – no risk of contamination
      • Usable for all know cell lines
      • Long shelf life
    • Categoría de Producto:
    • Medios de congelación
    • Capacidad/Volumen:
    • 1x120 ml
    • Temperatura Envío:
    • Refrigerado 4º
    • Temperatura Almacenaje:
    • Refrigerado 4º
    • Disponibilidad:
    • Disponible bajo pedido
    • Aplicaciones:
    • Bioprocesos
Documentos