Marcador de proteínas BlueStar Plus para electroforesis, 1 tubo con 500µl

Ref.: 523272
Cod. Prov: MWP04
Ref. Cultek: B4MWP04
143,22 € sin iva
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BlueStar PLUS Prestained Protein Marker

The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240
kDa. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

  • Broad range: 10 – 240 kDa
  • High performance: 12 bands with 3 colours (blue, red and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels
  • Easy to identify: The 25 (green) and 75 kDa (red) are reference bands
  • Sharp bands 

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store at -20°C for 12 months.
Stable at +4°C for 3 months.

Recommendations for Loading

  1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
  2. Mix thoroughly, to ensure that the solution is homogeneous.
  3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
    – 5 μl per well for mini-gels, 3 μl per well for blots;
    – 10 μl per well for large gels, 6 μl per well for blots

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Más Información
Envase 500 µl
Aplicaciones Electroforesis
Categoría de Producto Reactivos
Subcategoría de Producto Marcadores y ladders
Temperatura Envío Refrigerado 4º
Temperatura Almacenaje Congelado -20º
Disponibilidad Entrega inmediata
Caracteristicas
  • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
  • Monitoring of protein transfer onto membranes during Western blotting
  • Sizing of proteins on SDS-polyacrylamide gels and Western blots
Marca NIPPON GENETICS

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