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Cultek, S.L.U.
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U.
Camí del Mig, 125 2a Planta, Puerta 35
08302 – MATARÓ (Barcelona)(Spain) Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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Marcador de proteínas BlueStar para electroforesis, 1 tubo con 500µl (NIPPON GENETICS)

Marcador de proteínas BlueStar para electroforesis, 1 tubo con 500µl

  • 500 µl
  • -
  • Disponibilidad:
BlueStar Prestained Protein Marker (500 µl)
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Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

BlueStar Prestained Protein Marker

The BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins.

  • Broad range: 10 – 180 kDa
  • High performance: 10 bands with 3 colours (blue, orange and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels.
  • Reference bands: 28 kDa (green) and 75 kDa (orange)
  • Sharp bands 

Storage Buffer

20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol.

Storage

Stable at -20°C for 12 months.
Stable at +4°C for 3 months. 

Recommendations for Loading

1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

  • 5 μl per well for mini-gels, 3 μl per well for blots
  • 10 μl per well for large gels, 6 μl per well for blots
    • Referencia:
    • B4MWP03
    • Envase:
    • 500 µl
    • Caracteristicas:
      • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis
      • Monitoring of protein transfer onto membranes during Western blotting
      • Sizing of proteins on SDS-polyacrylamide gels and Western blots
    • Categoría de Producto:
    • Reactivos
    • Subcategoría de Producto:
    • Marcadores y ladders
    • Capacidad/Volumen:
    • 500 μl
    • Ofertas y novedades:
    • Oferta
    • Temperatura Envío:
    • Refrigerado 4º
    • Temperatura Almacenaje:
    • Congelado -20º
    • Disponibilidad:
    • Entrega inmediata
    • Marca:
    • NIPPON GENETICS
    • Aplicaciones:
    • Electroforesis
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