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Cultek, S.L.U.
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U.
Camí del Mig, 125 2a Planta, Puerta 35
08302 – MATARÓ (Barcelona)(Spain) Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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FastGene Taq Polimerasa para PCR directa, 500 unidades, 1 tubo (NIPPON GENETICS)

FastGene Taq Polimerasa para PCR directa, 500 unidades, 1 tubo

  • 500 u
  • -
  • Disponibilidad:
FastGene Taq DNA Polymerase (500 U)
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Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

FastGene DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme is purified using three different chromatography technologies and result in a very high purity and very high activity. 5 U/uL FastGene DNA Polymerase contain 150 ng/uL (+/- 10%) protein of >98 % purity. PCR products generated with FastGene DNA Polymerase are A-tailed and therefore compatible with TA cloning systems. Two different reaction buffers The enzyme comes with 2 different reaction buffers. Buffer A is a “high yield” buffer; for most amplicons, when you compare Buffer B and Buffer A, Buffer A will give higher yield. Buffer B is a standard KCl-based Taq buffer which we supply in case a customer wants to change only the enzyme, and keep the buffer the same. Furthermore we have seen a higher sensitivity by using Buffer B in some PCR assays. This is just a guideline, for some applications the reverse will be true, but generally, this is the trend that we’ve seen. The standardPackage size is 500 units and 2000 units - samples for testing are available (100 units). Shelf life is at least 12 months. The kit should be stored at -20 °C. Shipping or short-term storage (up to 3 days) can be done at room temperature. The kit includes the enzyme, two different reaction buffers (buffer A and B) as well as a separate tube of Magnesiumchloride, if the concentration of this salt needs to be higher than1.5 mM.
    • Envase:
    • 500 u
    • Caracteristicas:
    • High throughput PCR Amplification of low copy DNA templates Multiplex PCR Specific amplification of complex templates RT-PCR TA-cloning
    • Referencia:
    • B4LS21
    • Categoría de Producto:
    • Polimerasas PCR
    • Subcategoría de Producto:
    • Rutina
    • Temperatura Envío:
    • Refrigerado 4º
    • Temperatura Almacenaje:
    • Congelado -20º
    • Disponibilidad:
    • Disponible en 10 días laborables
    • Aplicaciones:
    • Amplificación (PCR y qPCR)
    • Marca:
    • NIPPON GENETICS