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Cultek, S.L.U.
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U.
Camí del Mig, 125 2a Planta, Puerta 35
08302 – MATARÓ (Barcelona)(Spain) Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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Kit de RT-qPCR Hi-ROX Probe de un paso para amplificación rápida de ARN, 500 reacciones (NIPPON GENETICS)

Kit de RT-qPCR Hi-ROX Probe de un paso para amplificación rápida de ARN, 500 reacciones

  • 500 rxns
  • -
  • Disponibilidad:
FastGene Probe One Step Mix Hi-Rox (500)
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Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

Applications

FastGene® Probe One Step mixes allow the performance of reverse transcription (RT) and qPCR in one reaction well. The big advantage is the direct amplification of RNA samples in the real time PCR instrument, what reduces the number of required sample manipulations and consequently operation time. Th mixes are ready-to-use with all necessary reagents required for the combined qPCR reaction and cDNA synthesis. FastGene® Probe One Step mixes are designed for use with probes, including TaqMan®, Scorpions® and molecular beacon probes.

  • Quantification of any RNA templates (e.g. mRNA, total RNA, viral RNA)
  • Detection of pathogens
  • High efficient detection of low copy number targets
  • High-throughput gene expression screening

Efficient and robust performance

In order to guarantee a great result for the combined reaction of cDNA synthesis and qPCR, the reverse transcriptase as well as the buffer chemistry were optimised according to the latest stand of knowledge. The modified transcriptase is characterized by a high efficiency and a thermal stability at temperatures from 45° – 55° C . An RNase inhibitor avoids DNA degradation by RNase. The hot start technology of the Taq DNA polymerase leads to high specific PCR reactions.

Robust chemistry for faster results

The FastGene® Probe buffers were designed to have a superior robustness. This guarantees the linearity of the qPCR and creates a better accuracy, essential for reproducible results. Additionally, qPCRs can be performed at lower amplifcation times for example using fast protocols

    • Envase:
    • 500 rxns
    • Referencia:
    • B4LS4705HR
    • Categoría de Producto:
    • Retrotranscriptasas
    • Subcategoría de Producto:
    • RT-PCR
    • Temperatura Envío:
    • Refrigerado 4º
    • Temperatura Almacenaje:
    • Congelado -20º
    • Disponibilidad:
    • Disponible en 15 días laborables
    • Aplicaciones:
    • Amplificación (PCR y qPCR)
    • Marca:
    • NIPPON GENETICS
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