Reactivos Silenciamiento Génico -ON-TARGETplus siRNA Set of 4 Upgrade- Human
Disponible
Guaranteed gene silencing and patented modifications to reduce off-targets; available as pre-designed ON-TARGETplus SMARTpool and siRNA reagents for human, mouse or rat.
Envase | 10 nmol |
---|---|
Aplicaciones | Silenciamiento génico |
Categoría de Producto | siRNA ON-TARGET plus |
Temperatura Envío | Ambiente |
Temperatura Almacenaje | Congelado -20º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Humano |
Especificaciones | Highlights Off-targets reduced by up to 90% compared to unmodified siRNA Guaranteed silencing by SMARTpool and 3 of 4 individual siRNAs (see Specifications tab) Sequence information provided with siRNA purchase A two-stranded mechanism requires a two-stranded solution Dharmacon scientists and collaborators demonstrated in 2006 (see Reference 1) that siRNA off-targets are mediated by antisense seed-region interactions, initiating the development of a dual-strand modification pattern: Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity Seed-region analysis on siRNA designs reduces miRNA-induced off-targets A landmark publication (see Reference 2) from the Dharmacon research group was the first to experimentally demonstrate the key role of the seed region in mediating off-targets. A subsequent 2008 paper (see Reference 3) showed the importance of seed frequency in the 3'UTR as in indicator of its likelihood to cause off-targets. These principles were subsequently applied to our siRNA designs to improve specificity: Design filters eliminate common seed regions likely to cause miRNA-like off-targets Seed frequency analysis for siRNA designs minimize off-target effects Available Product Formats SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus guaranteed to silence by 75% or better (see Specifications). Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene. Three of four siGENOME and ON-TARGETplus siRNAs are guaranteed to silence by 75% or better (see Specifications). Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene. Individual siRNAs: Select 1, 2, or 3 individual siRNAs per gene. Minimum purchase of 4 at the 2 nmol size. |
Caracteristicas | siRNA designed and modified for greater specificity |
Marca | HORIZON |
Recomendaciones
-
Reactivos Transfección -DharmaFECT 1 Transfection Reagent573,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control hCMV-None UHT 102.868,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenc. Génico SMARTvector Inducible DNA Damage Response Lentiviral shRNA Pooled Library mEF1a-GFP, Human6.852,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Expresión Génica -MGC Fully Sequenced Bovine cDNA (glycerol stock)130,42 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Gapdh Positive Control hEF1a_TurboGFP - Rat830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -Accell siRNA SMART Pool - Mouse739,24 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting mCMV-TurboGFP Control Particles 3830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Protein Kinase Lentiviral shRNA Pooled Library hEF1a-RFP - Human9.084,48 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -siGENOME siRNA RTF - Ubiquitin Conjugation Subset 3 - white plates - Mouse7.830,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible GAPDH Positive Control mEF1a_TurboGFP- Human830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -siGLO Lamin A_C Control siRNA - Human1.277,48 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -Set of 3 SMARTvector Lentiviral shRNA Glycerol - Rat1.163,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Kits Silenciamiento Génico -Trans-Lentiviral ORF Packaging Kit with Calcium Phosphate1.649,83 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Deubiquitinating Enzymes - Set of 4 - Human3.335,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting mCMV-TurboGFP Glycerol 6463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Transcription Factors Lentiviral shRNA Pooled Library mEF1a, RFP - Mouse9.084,48 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -Precision LentiORF Proteases5.153,59 € sin ivaPara ver tus precios y comprar inicia sesión
-
Estándar de referencia multiplex línea somática para BRCA I (formato FFPE) =400ng_pieza131,89 € sin ivaPara ver tus precios y comprar inicia sesión