Reactivos Silenciamiento Génico -Accell siRNA Set of 4 - Human

Cod. Prov: EQ-HUMAN-XX-0020
Ref. Cultek: 77EQ-HUMAN-XX-0020
1.777,48 € sin iva
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A novel siRNA for difficult-to-transfect cells, Accell siRNA is modified to require no transfection reagent or viral vector for delivery. Available as genome-wide, predesigned SMARTpool and single siRNA reagents for human, mouse, and rat.
Más Información
Envase 20 nmol
Aplicaciones Silenciamiento génico
Categoría de Producto Reactivos
Subcategoría de Producto siRNA Accell
Temperatura Envío Ambiente
Temperatura Almacenaje Refrigerado 4º
Formato Especial Grupo de 4
Disponibilidad Bajo pedido
Especie/Huesped Humano
Especificaciones Accell siRNA enters cells without the need for transfection reagents, virus (or viral vectors), or instruments Novel siRNA modifications facilitate uptake, stability, specificity and knockdown efficiency Proven performance in neuronal, immunological, primary, and other difficult-to-transfect cell types Appropriate for use in vivo, due to stabilizing modifications Extended-duration knockdown with optimized continuous application Experimental considerations This breakthrough in siRNA delivery requires no transfection reagent, but has some unique application requirements. Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended Full-serum media can be added back after 48 hours of incubation, optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown Product Formats SMARTpool: A mixture of 4 siRNA provided as a single reagent, providing advantages in both potency and specificity Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene Individual siRNAs: Select 1, 2, 3, or 4 individual siRNAs per gene Sequence information is provided with purchase of predesigned siRNA products
Caracteristicas Targeted gene silencing in difficult-to-transfect cells