Reactivos Expresión Génica -Precision LentiORF RFP Positive Control

Cod. Prov: OHS5832
Ref. Cultek: 77OHS5832
445,42 € sin iva
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Precision LentiORFs are human cDNA open reading frames (ORFs) cloned into a lentiviral expression vector, used for overexpressing human genes and proteins in mammalian cells
Más Información
Envase 1 x glycerol stock
Aplicaciones Expresión génica
Subcategoría de Producto LentiORFs
Temperatura Envío Congelado -80º
Temperatura Almacenaje Congelado -80º
Disponibilidad Bajo pedido
Capacidad/Volumen 2 ml
Especie/Huesped Mamífero
Especificaciones Highlights Lentiviral ORF constructs require no subcloning for mammalian expression Fully sequenced coding sequences GFP reporter is transported to the nucleusfor easy visualization of expression Clones are available with or without native stop codons Available as ready-to-transduce viral particles, glycerol stocks, or LentiORF Starter Kits Applications Potential applications of Precision LentiORFs include gene overexpression experiments, RNAi rescue (see the RNAi Rescue using Precision LentiORF technical note), and expression of human proteins in mammalian cell lines. Primary and non-dividing cells can be readily transduced with ready-to-use lentiviral particles transfected with Precision LentiORF constructs directly into your cell line. For generating your own viral particles, Trans-Lentiviral ORF packaging kits are also available. Formats Individual Clones– Glycerol Stock Pre-defined gene families- Arrayed glycerol stocks (includes with and without native stop clones) Complete genome-scale collection- Arrayed glycerol stocks(includes with and without native stop clones) Viral Particles– provided as 2x25µL aliquots with a minimum titer of 10^8TU/mL LentiORF Starter Kits– Choose from transfection kits, transduction kits, and viral particle kits Viral Particles– provided as 2x25µL aliquots with a minimum titer of 10^8TU/mL Precision LentiORF Viral Particle starter kits include: Two Precision LentiORF constructs provided as 2x25µL aliquots of high-titer viral particles (1x10^8TU/mL) Precision LentiORF RFP control provided as 25µL of high-titer virus Precision LentiORF Transduction starter kits include: Two Precision LentiORF constructs Trans-Lentiviral packaging mix sufficient for 10 packaging events Calcium Phosphate transfection reagent Precision LentiORF RFP control Note The Precision LentiORF expression vectors are not compatible with third generation packaging systems. We recommend the Trans-Lentiviral Packaging System for use with these vectors.
Caracteristicas Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter