Reactivos Expresión Génica -Edit-R CRISPRa Lentiviral mCMV-Blast-dCas9-VPR Particles

Cod. Prov: VCAS11920
Ref. Cultek: 77VCAS11920
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Edit-R CRISPRa Lentiviral mCMV-Blast-dCas9-VPR Particles
Más Información
Envase 50 µl 10^7 TU/ml
Aplicaciones Expresión génica
Categoría de Producto CRISPRa
Subcategoría de Producto Lentiviral
Temperatura Envío Congelado -80º
Temperatura Almacenaje Congelado -80º
Disponibilidad Bajo pedido
Especificaciones Highlights Selection using blasticidin resistance marker (BlastR) or fluorescent marker (mKate2 or TurboGFP™) Provided as concentrated, purified lentiviral particles for immediate transduction, with a minimum ≥ 1 × 107 TU/mL functional titer, by qPCR titering. Also available as certified endotoxin-free plasmid DNA for direct transfection into a packaging cell line and production of your own lentiviral particles Customize your construct with one of six SMARTchoice constitutive promoters to ensure optimal Cas9 expression in your cell line of interest Utilize the tight regulation of the inducible Cas9 vector when you require temporal control over the expression of Cas9 or to generate a stable cell line with minimal background expression. Not all RNA pol II promoters are equally active in different cellular environments The activity of any given promoter controlling the transcription of Cas9 nuclease can differ greatly from one biological context to another, resulting in variable Cas9 expression levels and thus varying levels of DNA cleavage. Choosing an optimal promoter for your cell line or type will therefore affect the degree of gene editing in your experiment. SMARTchoice promoter options for expressing Cas9 nuclease Promoter Description hCMV human cytomegalovirus immediate early promoter mCMV mouse cytomegalovirus immediate early promoter hEF1α human elongation factor 1 alpha promoter mEF1α mouse elongation factor 1 alpha promoter PGK mouse phosphoglycerate kinase promoter CAG chicken beta actin hybrid promoter TRE3G doxycycline-inducible promoter
Caracteristicas Lentiviral CRISPR-Cas9 components for robust gene editing in biologically relevant cell types