Reactivos Expresión Génica -Edit-R CRISPRa Lentiviral hEF1a-Blast-dCas9-VPR Plasmid DNA

Cod. Prov: CAS11916
Ref. Cultek: 77CAS11916
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The CRISPR activation (CRISPRa) system is a variation of the canonical CRISPR-Cas9 used for creation of double-strand breaks in genomic DNA. It utilizes a nuclease-deactivated S. pyogenes Cas9 (dCas9), often called "dead Cas9", that is fused to one or more transcriptional activators. When paired with a well-designed guide RNA that targets a gene near a promoter region, the gene's native transcription start site is activated. Edit-R Lentiviral dCas9-VPR Reagents are available with three different promoters (hCMV, mCMV, or hEF1a) and can be supplied as either purified high-titer lentiviral particles or purified plasmid DNA.
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Envase 10 µg
Aplicaciones Expresión génica
Categoría de Producto Cas9 lentiviral
Subcategoría de Producto Lentiviral (CRISPRa)
Temperatura Envío Ambiente
Temperatura Almacenaje Refrigerado 4º
Disponibilidad Bajo pedido
Especificaciones Choose from one of three different promoters for optimum expression in your cells of interest • Provided as concentrated, purified lentiviral particles for immediate transduction, with a minimum ≥ 1 × 107 TU/mL functional titer, by qPCR titering • Also available as certified endotoxin-free plasmid DNA for direct transfection into a packaging cell line and production of your own lentiviral particles • Selection using blasticidin resistance marker (BlastR) for entire cell populations or for clonal expansion of individual cells
Caracteristicas Purified lentiviral particles or plasmid DNA for generation of stable dCas9-VPR nuclease-expressing cell populations