Reactivos Edición Génica -Edit-R synthetic crRNA - Human

CRISPR-Cas9 is a highly effective tool for interrogating gene function, yet not all guide RNAs (gRNA) are effective in attaining functional protein knockout. To address this problem, Dharmacon developed an algorithm that is trained and validated for functional knockout, not just the ability to create double-strand breaks (DSB). With pre-designed CRISPR RNA (crRNA) you can quickly and easily assess multiple crRNA per gene, for many different genes. Edit-R crRNA is a chemically synthesized RNA, comprised of 20 nucleotides identical to the genomic DNA target site, or protospacer, followed by the required S. pyogenes repeat sequence that interacts with the tracrRNA, which is required for use with synthetic crRNA. The chosen 20-base target sequence in the gene is immediately upstream of a protospacer-adjacent motif (PAM) in the genomic DNA.