Reactivos Edición Génica -Edit-R mKate Transfection Optimization Plasmid

Cod. Prov: U-003000-120
Ref. Cultek: 77U-003000-120
Marca: HORIZON
187,77 € sin iva
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Endotoxin-free, purified DNA for direct co-transfection with Edit-R synthetic crRNA and tracrRNA. Choose from three options to facilitate enrichment of Cas9-expressing cells.
Más Información
Envase 120 µg
Aplicaciones Edición génica
Categoría de Producto Cas9 plásmido
Temperatura Envío Ambiente
Temperatura Almacenaje Refrigerado 4º
Disponibilidad Bajo pedido
Especificaciones There are three Edit-R Cas9 Nuclease Expression plasmid options from which to choose, based on preference of enrichment method and cell types: 1.Edit-R Cas9 Expression plasmids with mKate2: Cas9 nuclease and the mKate2 fluorescent reporter are both expressed under the control of a single RNA pol II promoter, making this plasmid useful for downstream cell enrichment by FACS. Offered with your choice of six different promoters. 2.Edit-R Cas9 Expression plasmids with PuroR: Cas9 nuclease and the Puromycin-resistance marker are both expressed under the control of a single RNA pol II promoter, making this plasmid useful for downstream cell enrichment by antibiotic (Puromycin) selection. Offered with your choice of six different promoters. 3.Edit-R Cas9 Nuclease Expression plasmid with hCMV-BlastR: Cas9 nuclease expression is driven from a human cytomegalovirus (hCMV) promoter, and Blasticidin resistance (BlastR) is under the control of the Simian virus 40 (SV40) promoter. This simple vector is useful for those who do not want a fluorescent protein constitutively expressed in the cells of interest and prefer to enrich for Cas9-expressing cells through Blasticidin treatment, especially if a longer antibiotic selection time is required. Not all RNA pol II promoters are equally active in different cellular environments The activity of any given promoter controlling the transcription of Cas9 nuclease can differ greatly from one biological context to another, resulting in variable Cas9 expression levels and thus varying levels of DNA cleavage. Choosing an optimal promoter for your cell line or type will therefore affect the degree of gene editing in your experimentation. The Edit-R Cas9 Nuclease expression plasmids are offered with six different, well-characterized cellular promoters from which you can choose.
Caracteristicas Non-lentiviral Cas9 expression plasmids with your choice of promoter and enrichment method
Marca HORIZON

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