qPCR DNA Extraction and Inhibition Control Yakima Yellow-BHQ1

Real-Time PCR assays are prone to inhibition by various substances found in many samples (clinical, soil, plant…). Carryover of reagents used for the isolation of nucleic acids can also inhibit amplification reactions. Other causes of false-negative results include target nucleic acid degradation, sample processing errors, and thermocycler malfunction.

Benefits

Specific

• Does not interfere with target amplification
• Avoid amplification of endogenous genes

Sensitive

• Compatible with low copy templates
• Available with dark quencher for maximal signal-to-noise ratio

Designed for multiplex assays

• Detected in the Yakima Yellow®, VIC®, JOE channel

Eurogentec’s Sample Processing Control provides an accurate way to assess the integrity of your nucleic acid extraction and amplification assay.

SPC are labelled to Yakima Yellow or Cy5, custom labelling is available on request (custom-qPCR@eurogentec.com).

The Sample Processing Control (SPC) is spiked into samples before extraction to monitor poor extraction yield, PCR inhibition, incorrect pipetting, or cycling parameters.

A given quantity of control can be spiked into samples before extraction. A relative (directly comparing samples) or an absolute (using a dilution curve of the control) quantification is performed after extraction to normalize the extraction yields of the samples.

Quantitative results of the spiked control within the template or within a reference buffer (pure water, reference template…) are compared in order to reject templates where PCR inhibition is high (low quality).

Add a dilution series of the optimised control on each plate and use it to normalise PCR efficiencies between plates (also for cycler to cycler data normalisation).