Mastering RNA interference: Essential guidelines and pro tips.

Webinar Rewity

 In this webinar, we dive deep into the mechanism of RNA interference (RNAi) and the use of small interfering RNA (siRNA) for gene silencing in experimental settings. We’ll walk you through the crucial steps of the endogenous RNAi pathway, providing a comprehensive context for how siRNA mediates targeted gene knockdown. 

Key factors that impact siRNA effectiveness and specificity, such as design, chemical modifications, and concentration, will be discussed in detail, with a strong emphasis on minimizing off-target effects. 

Additionally, we’ll cover best practices for designing siRNA-based experiments, including the use of positive and negative controls, transfection optimization, and determining the optimal siRNA concentration. We’ll also address strategies for effective siRNA delivery, particularly in challenging cell types that are difficult to transfect. 

By the end of this session, participants will have a thorough understanding of: 

• The essential steps in the endogenous RNAi pathway.
• Consideration for siRNA silencing functionality and specificity.
• Best practices for designing and executing successful siRNA experiments.