Increasing CRISPR knockout and screening efficiency with predesigned synthetic guide RNAs
CRISPR-based genome editing has accelerated biological research and offers immense potential for studying and treating human diseases. The CRISPR-Cas9 system requires a Cas9 nuclease and a guide RNA, which can consist of either a CRISPR RNA (crRNA) coupled with a trans-activating crRNA (tracrRNA), or a single guide RNA (sgRNA) which...