Mix de ligación de ADN FastGene Kickspeed 2x (50 rxns)

Kickspeed DNA Ligation Kit

FastGene® Kickspeed DNA Ligation kit is formulated for quick ligation of DNA fragments with cohesive ends within 5 min at room temperature. This product uses T4 DNA Ligase and 2X Kickspeed DNA Ligation buffer.

Characteristics

• 5 min ligation reaction with all types of DNA fragments
• Reaction at room temperature
• Rapid and efficient Kickspeed Ligation buffer

Applications

• General cloning
• Blunt-end cloning
• TA cloning
• Library construction
• Linker ligation

T4 DNA Ligase

The FastGene® T4 DNA Ligase catalyzes the formation of a covalent bond between the 5′-phosphate and 3′-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends, that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. The T4 DNA Ligase is cloned and expressed in E. coli, and purified to homogeneity. The Ligase is free of endonuclease, exonuclease and phosphatase.