Librería Silenciamiento Génico -SMARTvector Phosphatase Lentiviral shRNA Pooled Library hEF1a, GFP - Mouse

Cod. Prov: V3SM8593
Ref. Cultek: 77V3SM8593
5.710,95 € sin iva
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Combine the advantages of rationally designed shRNA with selectable promoters and reporters to perform functional screens of hundreds or thousands of shRNAs in dividing and non-dividing cells.
Más Información
Envase 200 µl, 10^8 TU/ml
Aplicaciones Silenciamiento génico
Categoría de Producto Librerías shRNA
Subcategoría de Producto SMARTvector
Temperatura Envío Congelado -80º
Temperatura Almacenaje Congelado -80º
Disponibilidad Bajo pedido
Especie/Huesped Ratón
Especificaciones Innovative vector design for robust shRNA expression from SMARTvector Lentiviral shRNAs SMARTchoice options give you a choice of promoter and reporter (or no reporter!) for optimal performance in your experiment Rationally designed shRNAs for maximum knockdown and minimal off-targeting Choose SMARTvector Inducible Lentiviral shRNA for tight regulation of expression from the Tet-On® 3G transactivator protein Each SMARTvector Lentiviral shRNA Library contains ≥ 5 x 108 TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs 16 x 25 µL (400 µL total) for libraries > 5000 constructs 100 Non-targeting control constructs 272 constructs targeting 34 human or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes. A data file containing construct sequences, target gene IDs, and counts per millions of mapped reads SMARTvector Lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom ​SMARTvector Lentiviral shRNA collections are available upon request. Request a custom collection of mouse mouse, or rat gene-targeting shRNAs Available in pool sizes between 50 to 10,000 constructs Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors Request pricing for a custom pooled library Products recommended in our validated protocol: Vector-matched SMARTvector Non-targeting control for transduction optimization SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias High-throughput multiplexed Next Gen Sequencing for hit identification Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required: SMARTvector & shMIMIC Lentiviral Pooled Libraries Technical Manual Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet SMARTvector Lentiviral shRNA pooled library coverage*: *Annotations are based on RefSeq v65; coverage and shRNA counts are subject to change without notice ** Clone and gene counts available by request Collection Human Mouse # targeted genes Number pools and average shRNA per pool # targeted genes Number pools and average shRNA per pool Genome 19241 16 pools of 9513 shRNA 21745 18 pools of 9502 shRNA Druggable Genome 7341 6 pools of 9735 shRNA 9723 8 pools of 9670 shRNA GPCR 377 1 pool of 3012 shRNA 494 1 pool of 3909 shRNA Ion Channel 340 1 pool of 2709 shRNA 332 1 pool of 2646 shRNA Phosphatase 245 1 pool of 1948 shRNA 268 1 pool of 2141 shRNA Protease 466 1 pool of 3702 shRNA 529 1 pool of 4206 shRNA Protein Kinase 702 1 pool of 5602 shRNA 697 1 pool of 5558 shRNA Ubiquitin Conjugation 557 1 pool of 4431 shRNA 511 1 pool of 4069 shRNA Apoptosis ** Cell Cycle Regulation ** De-ubiquitinating Enzymes ** Membrane Trafficking ** DNA Damage Response ** Epigenetics ** Nuclear Receptor ** Transcription Factors ** Important Notice The SMARTvector Lentiviral shRNA Pooled Libraries are solely for internal research use (as set forth in the Product Terms and Conditions) in laboratories where the containment measures stated below and in applicable laws and regulations are met. Products may not be used for diagnostic, therapeutic or other commercial purposes and may not to be administered to humans for any purpose or to animals for therapeutic purposes. SMARTvector Lentiviral shRNA Pooled Libraries are provided as lentiviral particles are replication-incompetent, self-inactivating (SIN) and non-pathogenic (do not cause infectious human disease). Any investigator who purchases lentiviral particle products is responsible for consulting with their institution's health and biosafety personnel for specific guidelines on the handling of lentiviral vector particles. Furthermore, each investigator is fully responsible for obtaining the required permissions for research using and the acceptance of replication-incompetent SIN lentiviral vectors and replication-defective lentiviral particles into their local jurisdiction and institution.
Caracteristicas Constitutive shRNA expression for optimized functional analysis screens