Librería Silenc. Génico SMARTvector Inducible Ubiquitin Conjugation Lentiviral shRNA Pooled Library mEF1a, RFP, Mouse
Disponible
Combine the advantages of rationally designed shRNAs and the flexibiltiy of selectable promoters and reporters with the tight control of the Tet-On 3G tetracycline-inducible transactivator for unmatched shRNA expression
Envase | 200 µl, 10^7 TU/ml |
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Aplicaciones | Silenciamiento génico |
Categoría de Producto | Librerías shRNA |
Subcategoría de Producto | SMARTvector |
Temperatura Envío | Congelado -80º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Ratón |
Especificaciones | Innovative vector design for robust inducible shRNA expression from SMARTvector Inducible Lentiviral shRNA SMARTchoice configuration options give you a choice of four inducible promoters and two fluorescent reporters for optimal performance in your experiment Tightly controlled regulation of shRNA expression from the Tet-On® 3G transactivator protein Choose SMARTvector Lentiviral shRNA Pooled Libraries for constitutive shRNA expression. Each SMARTvector Inducible Lentiviral shRNA Library contains: ≥ 1 x 107 TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs 16 x 25 µL (400 µL total) for libraries > 5000 constructs 100 Non-targeting control constructs 272 constructs targeting 34 human or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes. A data file including construct sequences, target gene IDs, and counts per millions of mapped reads SMARTvector Inducible Lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom SMARTvector Inducible Lentiviral shRNA collections are available upon request. Request a custom collection of human, mouse, or rat gene-targeting shRNAs Available in pool sizes between 50 to 10,000 constructs Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors Request pricing for a custom pooled library Products recommended in our validated protocol: Vector-matched SMARTvector Inducible Non-targeting control for transduction optimization SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias High-throughput multiplexed Next Gen Sequencing for hit identification Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required: SMARTvector & shMIMIC Lentiviral Pooled Libraries Technical Manual Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet |
Caracteristicas | Inducible shRNA expression for regulatable and controlled functional analysis screens |
Marca | HORIZON |
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