Librería Silenc. Génico SMARTvector Inducible DNA Damage Response Lentiviral shRNA Pooled Library mCMV-GFP, Human
Disponible
Combine the advantages of rationally designed shRNAs and the flexibiltiy of selectable promoters and reporters with the tight control of the Tet-On 3G tetracycline-inducible transactivator for unmatched shRNA expression
Envase | 200 µl, 10^7 TU/ml |
---|---|
Aplicaciones | Silenciamiento génico |
Categoría de Producto | Librerías shRNA |
Subcategoría de Producto | SMARTvector |
Temperatura Envío | Congelado -80º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Humano |
Especificaciones | Innovative vector design for robust inducible shRNA expression from SMARTvector Inducible Lentiviral shRNA SMARTchoice configuration options give you a choice of four inducible promoters and two fluorescent reporters for optimal performance in your experiment Tightly controlled regulation of shRNA expression from the Tet-On® 3G transactivator protein Choose SMARTvector Lentiviral shRNA Pooled Libraries for constitutive shRNA expression. Each SMARTvector Inducible Lentiviral shRNA Library contains: ≥ 1 x 107 TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs 16 x 25 µL (400 µL total) for libraries > 5000 constructs 100 Non-targeting control constructs 272 constructs targeting 34 human or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes. A data file including construct sequences, target gene IDs, and counts per millions of mapped reads SMARTvector Inducible Lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom SMARTvector Inducible Lentiviral shRNA collections are available upon request. Request a custom collection of human, mouse, or rat gene-targeting shRNAs Available in pool sizes between 50 to 10,000 constructs Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors Request pricing for a custom pooled library Products recommended in our validated protocol: Vector-matched SMARTvector Inducible Non-targeting control for transduction optimization SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias High-throughput multiplexed Next Gen Sequencing for hit identification Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required: SMARTvector & shMIMIC Lentiviral Pooled Libraries Technical Manual Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet |
Caracteristicas | Inducible shRNA expression for regulatable and controlled functional analysis screens |
Marca | HORIZON |
Recomendaciones
-
Reactivos Transfección -DharmaFECT 1 Transfection Reagent573,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Estándar de referencia Tru-Q 1 (5_ Tier)(formato gDNA) 50ng_µl, 1µg287,40 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Ion Channels - SMARTpool - Mouse12.013,89 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control mCMV-None UHT 92.868,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Inducible Protease Lentiviral shRNA Pooled Library mEF1a-GFP - Human6.852,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -ON-TARGETplus siRNA Set of 4 Upgrade- Human0,00 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Ppib Positive Control hEF1a-TurboGFP - Rat Glycerol - Rat463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -siGENOME siRNA individual - Rat261,30 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting mCMV-None Control Particles 12830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Nuclear Receptor Lentiviral shRNA Pooled Library hEF1a-GFP - Human5.710,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -siGENOME siRNA RTF - Membrane Trafficking - Mouse2.387,42 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -shMIMIC Lentiviral microRNA -Human1.195,42 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -siGLO RISC-Free Control siRNA289,24 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Inducible GPCR Lentiviral shRNA Pooled Library PGK-RFP - Mouse6.852,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Transfección -DharmaFECT Kb Transfection Reagent338,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting PGK-TurboRFP Glycerol 4463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector De-ubiquitinating Enzymes Lentiviral shRNA Pooled Library mEF1a, RFP, Mouse5.710,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -Precision LentiORF Cytokine Receptors2.860,95 € sin ivaPara ver tus precios y comprar inicia sesión