Librería Silenc. Génico SMARTvector Inducible Cell Cycle Regulation Lentiviral shRNA Pooled Library hEF1a-GFP, Human
Disponible
Combine the advantages of rationally designed shRNAs and the flexibiltiy of selectable promoters and reporters with the tight control of the Tet-On 3G tetracycline-inducible transactivator for unmatched shRNA expression
Envase | 200 µl, 10^7 TU/ml |
---|---|
Aplicaciones | Silenciamiento génico |
Categoría de Producto | Librerías shRNA |
Subcategoría de Producto | SMARTvector |
Temperatura Envío | Congelado -80º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Humano |
Especificaciones | Innovative vector design for robust inducible shRNA expression from SMARTvector Inducible Lentiviral shRNA SMARTchoice configuration options give you a choice of four inducible promoters and two fluorescent reporters for optimal performance in your experiment Tightly controlled regulation of shRNA expression from the Tet-On® 3G transactivator protein Choose SMARTvector Lentiviral shRNA Pooled Libraries for constitutive shRNA expression. Each SMARTvector Inducible Lentiviral shRNA Library contains: ≥ 1 x 107 TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs 16 x 25 µL (400 µL total) for libraries > 5000 constructs 100 Non-targeting control constructs 272 constructs targeting 34 human or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes. A data file including construct sequences, target gene IDs, and counts per millions of mapped reads SMARTvector Inducible Lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom SMARTvector Inducible Lentiviral shRNA collections are available upon request. Request a custom collection of human, mouse, or rat gene-targeting shRNAs Available in pool sizes between 50 to 10,000 constructs Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors Request pricing for a custom pooled library Products recommended in our validated protocol: Vector-matched SMARTvector Inducible Non-targeting control for transduction optimization SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias High-throughput multiplexed Next Gen Sequencing for hit identification Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required: SMARTvector & shMIMIC Lentiviral Pooled Libraries Technical Manual Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet |
Caracteristicas | Inducible shRNA expression for regulatable and controlled functional analysis screens |
Marca | HORIZON |
Recomendaciones
-
Controles Edición Génica -Edit-R crRNA Non-targeting Control #3156,89 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Tyrosine Kinases - Set of 4 - Mouse4.953,59 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting mCMV-TurboGFP Glycerol 10463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -Accell siRNA individual - Mouse592,18 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector DNA Damage Response Lentiviral shRNA Pooled Library mCMV - Mouse5.710,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -ORFeome Collaboration Mouse Clone-w_o Native Stop Codon (glycerol stock)513,89 € sin ivaPara ver tus precios y comprar inicia sesión
-
Control #2 sgRNA sintético letal Edit-R431,89 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Epigenetics - SMARTpool - Mouse -384 well20.218,30 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting hCMV-TurboGFP Glycerol 3463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Inducible Druggable Lentiviral shRNA Pooled Library PGK-RFP - Human13.553,59 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -MGC Fully Sequenced Human cDNA (pCMV) Set Protein Kinase (glycerol stock)2.441,83 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -Yeast Essential Collection (glycerol stock)978,59 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting mCMV-TurboGFP Control Particles 8830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Transcription Factors Lentiviral shRNA Pooled Library mEF1a-RFP - Human9.084,48 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -ON-TARGETplus RTF - Ubiquitin Conjugation Subset 1 - Human2.300,65 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -shMIMIC Lentiviral microRNA Pooled Library - Human-Mouse Conserved mCMV-GFP4.025,65 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -ON-TARGETplus Cyclophilin B Control siRNA - Human208,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Expresión Génica -Edit-R CRISPRa Lentiviral hEF1a-Blast-dCas9-VPR Particles673,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Expresión Génica -Edit-R Ttn CRISPRa crRNA control - Mouse156,89 € sin ivaPara ver tus precios y comprar inicia sesión