Kit de RT-PCR con transcriptasa reversa Ultrascript 2.0, 25rxns, Ultrascript 1x25µl, mix reacción 1x100µl

Cod. Prov: PB30.31-02
Ref. Cultek: K7PB30.31-02
162,35 € sin iva
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UltraScript 2.0 Reverse Transcriptase is a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed,yield and representation from a wide range of RNA sample types,including GC-rich and low abundance templates. The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used,giving improved specificity,higher cDNA yields,and more full length cDNA product. 

For RT-qPCR applications,the 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast,reliable and unbiased cDNA synthesis,including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes,for user optimisation depending on the type of analysis needed. 

UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is pre-blended with an advanced RNase inhibitor to prevent degradation of RNA by contaminating RNase.

Más Información
Envase 25 rxns
Aplicaciones Amplificación (PCR y qPCR)
Categoría de Producto Retrotranscriptasas
Subcategoría de Producto MMLV
Temperatura Envío Congelado -20º
Temperatura Almacenaje Congelado -20º
Disponibilidad Entrega inmediata
  • Highly thermostable reverse transcriptase
  • 55°C to 65°C and above
  • Advanced RNase inhibitor
  • High cDNA yields from as little as 20pg total RNA
  • Accurate reverse transcription of GC-rich and highly structured transcripts
  • Sensitive detection of low copy number transcripts
  • Reduced RNase H activity
  • Advanced buffer chemistry including Mg and
  • dNTPs
  • Available as a stand-alone enzyme with buffer,a cDNA synthesis kit with premixed oligos optimised for qPCR analysis,and a cDNA synthesis kit with separate oligos