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Cultek, S.L.U.  MADRID
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) 
Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U. BARCELONA
Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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Transcriptasa reversa FastGene Scriptase II para RT-PCR, 100 reacciones (NIPPON GENETICS)

Transcriptasa reversa FastGene Scriptase II para RT-PCR, 100 reacciones

  • 100 rxns
  • -
  • Disponibilidad:
FastGene Scriptase II cDNA 5x ReadyMix (100 rxns)
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Disponibilidad: Entrega inmediata

Descripción

Descripción & Documentación

Ready Mix

The FastGene® Scriptase II 5x ReadyMix is an engineered enzyme with a reduced RNase H activity and comesready-to-use with all necessary ingredients to perform a revers transcription.

 

Engineered enzyme

Engineered reverse transcriptases allowed the synthesis of cDNA from very low amounts of RNA. Mutations are inserted into the RNase H domain of the MuLV‘s reverse transcriptase. Therefore, by not degrading the RNA during the first-strand synthesis, a higher yield of full-length cDNA is obtained. Additionally, a higher thermal stability increases the robustness of the enzyme. The FastGene® Scriptase II is exactly one of those engineered enzymes. With its mutation in the RNase H domain and higher thermal stability, it is the optimal choice for more complex applications, such as RT-qPCR and NGS.

 

Lower RNase H activity for longer cDNA

The FastGene® Scriptase II has a modified RNase H domain. The RNA is therefore not degraded and serves as a template for longer cDNAs, resulting in fragment size of up to 12 kBp.

 

Engineered enzymes – optimized for qPCR

The FastGene® Scriptase II delivers superior cDNA templates for downstream applications, e.g. qPCR and NGS. The resulting full-length cDNA gives a complete picture of the gene and is able to show modification, e.g. splicing variants.

The FastGene® Scriptase II 5x ReadyMix comes with all necessary ingredients to perform a revers transcription:

  • FastGene® Scriptase II
  • 5x FastGene® Scriptase II buffer
  • dNTP Mixture
  • RNase Inhibitor
  • Random Hexamer
  • Helper Protein
    • Referencia:
    • B4LS64
    • Especificaciones:
    • 1. Mix the ReadyMix with the RNA Template using the following setup:

      5x FastGene® Scriptase II ReadyMix (LS64) 4 µl
      High quality RNA-template* x µl
      Sterile Water up to 20 µl

      *Concentration of template RNA can be up to 2.5 μg

       

      2. Incubate at 25 °C for 10 minutes.

      3. Incubate at 42 °C for 60 minutes*.

      *Note: The recommended incubation time can be reduced to 5 minutes depending on the product abundance and size.

       

      4. Inactivate the FastGene Scriptase II by incubating at 85°C for 5 min.

      5. Store the cDNA at -20 °C or use it for downstream application.

    • Categoría de Producto:
    • Retrotranscriptasas
    • Subcategoría de Producto:
    • RT-PCR
    • Temperatura Envío:
    • Congelado -20º
    • Temperatura Almacenaje:
    • Congelado -20º
    • Disponibilidad:
    • Entrega inmediata
    • Aplicaciones:
    • Amplificación (PCR y qPCR)
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