Estándar de referencia PI3KCA E542K 50_ (formato gDNA) 50ng_µl, 1µg

Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies.

Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

Technical Data

• DNA Base Change GAA→AAA NCBI Reference Assembly SNP rs121913273
• Cosmic ID COSM760
• Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.74

Product Information

• Intended Use For routine performance monitoring (Research Use Only)
• Unit Size 1 µg Concentration 50ng/µl

General Information

• Allelic Frequency 50%
• Storage 4˚C
• Expiry 36 months from the date of manufacture
• Cell Line Background SW48

Quality Control

• Allelic Frequency Droplet Digital PCR™ Genotype Sanger sequencing of locus specific PCR Quality Agarose gel electrophoresis Quantification Spectrophotometry (A260) Genotype PIK3CA (E542K/+)Product information- please check in the Horizon webpage https://www.horizondiscovery.com