Estándar de referencia multiplex específico de EGFR (formato FFPE) 1_ frecuencia alélica,=400 ng_pieza

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Gene-Specific Multiplex Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions at defined allelic frequency ranges. The Gene-Specific Reference Standards cover EGFR or KRAS-specific mutations. These standards may be diluted with a matched wild type while the background mutations remain consistent. The Gene Specific Multiplex Standards are available in genomic DNA and FFPE format, which allows you to validate both the pre-analytical and analytical portion of your workflow. The FFPE format allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples.

By multiplexing multiple gene-specific mutations in a single standard, the Gene-Specific Multiplex allows researchers to maximize the information obtained from their assay, and confirm that they are able to detect a range of potential mutants in a single gene.

Technical Data

• Genes Covered EGFR Cosmics 950+ Fixation Method 4%
• Formalin Section Size 15µm or 20µm
• Cell Density 3 x 108 cells per block.
• Approx. 3.5 x 105 cells per section
• Expected DNA Yield ≥ 400 ng using Promega Maxwell LEV Plus Extraction kit
• Product information- please check in the Horizon webpage https://www.horizondiscovery.com