Controles Silenciamiento Génico -SMARTvector Inducible Gapdh Positive Control PGK-TurboRFP - Mouse Glycerol - Mouse
Disponible
The SMARTvector Inducible RNAi Controls are cost-effective tools for determining optimal conditions for delivery prior to using gene-specific SMARTvector Inducible Lentiviral shRNAs and shMIMIC Inducible Lentiviral microRNAs
Envase | 1 x glycerol stock |
---|---|
Aplicaciones | Silenciamiento génico |
Categoría de Producto | Controles shRNA |
Subcategoría de Producto | Positivo |
Temperatura Envío | Congelado -80º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Especie/Huesped | Ratón |
Especificaciones | Start by identifying the optimal vector configuration for your cells with the SMARTchoice Promoter Selection Plate Determine the most active promoter in the specific cells of interest by evaluating all seven vectors simultaneously using the SMARTchoice Promoter Selection Plate (catalog # SP-001000-01): An arrayed plate of SMARTvector Non-targeting Controls (NTCs) with seven well-characterized constitutive cellular promoters driving the expression of TurboGFP (Evrogen, Moscow, Russia) High-titer lentiviral particles are packaged, purified, and concentrated for consistent activity Lentiviral particles are arrayed in a convenient 96- well format in duplicate serial dilutions so multiple MOIs can be tested. Order SMARTvector RNAi controls with promoter and reporter selections matching your gene-specific constructs. SMARTvector vector-matched controls are available with options for all seven promoters and two fluorescent reporters or without a fluorescent reporter. All individual controls are available as glycerol stocks and as 50 µL (2 x 25 µL) of 1 x 108 TU/mL, ± 20%, and 100 µL (4 x 25 µL) of 5 x 109 TU/mL, ± 20%, of purified, concentrated packaged lentiviral particles. SMARTvector Non-targeting Controls Validated to have minimal targeting of any known genes in the human, mouse and rat genomes Confirm transduction efficiency via TurboGFP or TurboRFP expression Distinguish sequence-specific silencing of gene-targeting SMARTvector shRNAs and shMIMIC microRNAs from non-specific effects Experimental considerations: SMARTvector Non-targeting Control 1: Our longest standing and most commonly used SMARTvector non-targeting control, ideal for supporting most experiments involving only a few genes SMARTvector Non-targeting Control 2 – 12: Designed specifically with the needs of screeners in mind, who may require using several different non-targeting control sequences in follow-up experiments; should be selected if multiple controls are desired SMARTvector Positve Controls Provide consistent, potent silencing of non-essential, abundantly expressed housekeeping genes in human (see Figure 2), mouse and rat cells SMARTvector GAPD Positive Control shRNA (human, mouse and rat); validated to target glyceraldehyde-3-phosphate dehydrogenase (also known as GAPDH) SMARTvector PPIB Positive Control shRNA (human, mouse and rat); validated to target peptidylprolyl isomerase B (also known as cyclophilin B) |
Caracteristicas | Matched Controls enable accurate data interpretation for both SMARTvector Inducible shRNA and shMIMIC Inducible microRNAs |
Marca | HORIZON |
Recomendaciones
-
Reactivos Transfección -DharmaFECT 1 Transfection Reagent573,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Transfección -DharmaFECT 3 Transfection Reagent573,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenc. Génico SMARTvector Inducible De-ubiquitinating Enzymes Lentiviral shRNA Pooled Library PGK-GFP, Human6.852,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -MGC Fully Sequenced Human cDNA (pCMV) Set Cell Cycle (glycerol stock)3.410,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD mEF1a Control Particles - Rat830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -Accell siRNA SMART Pool - Rat1.903,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting hEF1a-TurboGFP Control Particles 5830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Whole Genome Lentiviral shRNA Pooled Library mEF1a-GFP - Human15.230,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -ON-TARGETplus RTF - GPCR - Human9.865,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector GAPD Control shRNA hEF1a-RFP UHT - Human2.868,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -siSTABLE Cyclophilin B Control siRNA - Mouse253,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Silenciamiento Génico -Set of 3 SMARTvector LNC shRNA 100 µL - Rat2.276,30 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Edición Génica -Edit-R CAG-PuroR-Cas9 Expression Plasmid DNA228,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Ubiquitin Conjugation Subset 3 - Set of 4 - Human16.327,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Inducible Non-targeting hEF1a-TurboGFP Glycerol 8463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -SMARTvector Ubiquitin Conjugation Lentiviral shRNA Pooled Library mCMV - Mouse5.710,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Expresión Génica -hORFeome v8.1 Nuclear Receptors1.274,18 € sin ivaPara ver tus precios y comprar inicia sesión
-
Control positivo, sgRNA lnetiviral de ratón, Edit-R Mouse All-in-one Lentiviral sgRNA430,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Librería Silenciamiento Génico -ON-TARGETplus siRNA Library - Ubiquitin Conjugation Subset 3 - SMARTpool - Mouse9.605,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Silenciamiento Génico -SMARTvector Non-targeting Control hEF1a-None UHT 122.868,95 € sin ivaPara ver tus precios y comprar inicia sesión