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Cultek, S.L.U.
Av. Cardenal Herrera Oria, 63
28034 Madrid (Spain) Tel. Centralita: +34 917 290 333
Tel. At. Cliente:  +34 902 04 4000 
Fax:   34 91 358 17 61
E-mail: info@cultek.com
Cultek, S.L.U.
Camí del Mig, 125 2a Planta, Puerta 35
08302 – MATARÓ (Barcelona)(Spain) Tel. Centralita:+34 938 611 660
Tel. At. Cliente:+34 902 04 4000
Fax:+34 93 860 03 53
E-mail: cultekbarna@cultek.com

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Verifi Polimerasa de alta fidelidad, long range, con colorante rojo para carga directa en gel (PCR BIOSYSTEMS)

Verifi Polimerasa de alta fidelidad, long range, con colorante rojo para carga directa en gel

  • 500 rxns
  • -
  • Disponibilidad:
    • Cod. Prov: PB10.44-05
    • -
    • Ref. Cultek: K7PB10.44-05
    • -
    • Marca: PCR BIOSYSTEMS
Polimerasa de alta fidelidad, long range, con colorante rojo para carga directa en gel
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Descripción & Documentación

Polimerasa de alta fidelidad, long range, con colorante rojo para carga directa en gel.

PCRBIO VeriFi Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required, such as cloning, site-directed mutagenesis and sequencing.

The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity, and is engineered with proprietary mutations that improve DNA binding and increase processivity when compared with its native form. This results in shorter extension times (10-30 seconds per kb), higher yields and the ability to amplify longer and more difficult targets. PCRBIO VeriFi Polymerase is able to amplify eukaryotic genomic templates in excess of 17.5kb, and longer for simpler DNA templates.

PCRBIO VeriFi Polymerase is provided with an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required. The enzyme is also available as a convenient 2x ready mix with the option of a red dye for direct gel loading, saving time during reaction setup and analysis. PCR products generated with this range of products are blunt ended.

    • Envase:
    • 500 rxns
    • Caracteristicas:
      • 100x higher fidelity than Taq DNA polymerase
      • Increased PCR success rates with complex genomic templates 17.5kb and over
      • Efficient, specific amplification from challenging templates including GC and AT rich sequences
      • High yields under standard and fast PCR conditions (10-30s/kb)
      • High temperature cycling for reduced GC bias
      • Available as a red mix for direct loading onto agarose gels
    • Referencia:
    • K7PB10.44-05
    • Categoría de Producto:
    • Ready Mix PCR
    • Subcategoría de Producto:
    • HiFi
    • Ofertas y novedades:
    • Oferta
    • Temperatura Envío:
    • Refrigerado 4º
    • Disponibilidad:
    • Disponible en 15 días laborables
    • Aplicaciones:
    • Amplificación (PCR y qPCR)
    • Marca:
    • PCR BIOSYSTEMS
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