Cultek S.L.U

Principios básicos de la purificación de ácidos nucleicos.

FAQs (Frequently Asked Questions) sobre:

	Extracción y purificación de DNA plasmídico.
	Extracción y purificación de DNA de geles.
	Extracción y purificación de RNA y/o mRNA.
	Extracción y purificación de DNA genómico.
	Extracción y purificación de ácidos nucleicos de virus.

Aplicaciones sobre extracción y purificación de ácidos nucleicos:

	Whole RNA isolation using NucleoSpin® 96 RNA on the AVISO Theonyx. (1.110 kB)
	Automated Total RNA Isolation from Cells and Tissue on the Hamilton MICROLAB® STAR using NucleoSpin® 96 RNA. (141 kB)
	Fully automated Genomic DNA Isolation from Blood on the Hamilton MICROLAB® STAR using NucleoSpin® 96 Blood kit. (119 kB)
	Automated PCR product purification with CyBi® -Genomics Workstation using NucleoFast® 96 PCR Plates. (39 kB)
	Fully Automated Purification of Genomic DNA from Tissue using the NucleSpin® 96 Plant HC Kit on the Multiprobe® II HT EX Platform with integrated Robotic Centrifuge. (998 kB)
	Fully Automated Purification of Genomic DNA from Plant using the NucleSpin® 96 Plant HC Kit on the Multiprobe® II HT EX Platform with integrated Robotic Centrifuge. (757 kB)
	Fast Automated PCR Purification with Ultrafiltration Technology on the MICROLAB® STAR. (117 kB)
	NucleoSpin® 96 Plant on the Tecan Freedom EVO. (249 kB)
	Automated purification of RNA on the Tecan Freedom Nucleic Acid Sample Preparation Workstation. (389 kB)
	Automated purification of PCR products with NucleoFast 96 PCR on the Tecan Freedom Nucleic Acid Sample Preparation Workstation. (703 kB)
	Automated purification of PCR products on the MWG RoboPrep® 2500 and the MWG RoboSeq® 4204 with the NucleoFast 96 PCR Kit. (1.153 kB)
	Automated RNA Purification on Tecan TeMo96. (201 kB)
	Automated Plasmid Purification on the Hamilton MICROLAB® STAR. (105 kB)
	Automated PCR Clean-Up with Silica on the Hamilton MICROLAB® STAR. (113 kB)
	Automated purification of total RNA on the MWG RoboSeq4204 with the NucleoSpin® 96 RNA kit. (677 kB)
	NucleoFast 96 PCR Purified DNA for Single Nucleotide Polymorphism Analysis Using the NanoChip® Technology (Nanogen). (445 kB)
	Use of the MN Wash Plate. (397 kB)
	NucleoSpin® Robot-96 Plasmid on BioRobot 8000 (Qiagen). (376 kB)
	NucleoSpin® Robot-96 Plasmid on BioRobot 9600 (Qiagen). (273 kB)
	Automated High Throughput 384 Well Plasmid Prep Using Corning FiltrEX Filter Plates.
	High Throughput Genomic And Proteomic Sample Preparation Using Corning FiltrEX 96 And 384 Well Filter Plates
	High Throughput PCR Cleanup Using 384 Well Glass Fiber Filter Plates And Positive Pressure On An Automated Workstation
	High Throughput Plasmid DNA Preparation Using PVDF Filter Plates On An Automated Workstation
	Rapid Efficient Purification of Both Plasmid and PCR DNA Using Corning Glass Fiber Filter Plates
	Plasmid Preparation Using Corning FiltrEX 384 Well Filter Plates
	Recovery of PCR Products Using FiltrEX 384 Well Glass Fiber Filter Plates
	Spin-X® Purification Of DNA From Agarose Gels
	Purification of PCR Products In Corning 96 Well Filter Plate.
	Purification of Plasmid DNA Using Corning Glass Fiber Filter Plates.

Referencias y bibliografía.

	Comprehensive DNA-Probe Assay Bibliography.
	Benchmarking 384 Well Plates For High Throughput PCR.
	Technical Parameters For The Use of Corning DNA-BIND Products In High-Throughput Screening.
	Thermowell® Sealing Mats.

Guías de selección de productos y plástico para PCR

	Corning Genomics Selection Guide.
	Corning Thermowell® GOLD PCR Microplates Compatibility Guide.
	Thermal Cycler Compatibility.

Propiedades de oligonucleótidos:

	Zuker mfold
	IDT OligoAnalyzer

Diseño de oligonucleótidos:

	Eurogentec: - Pdf - Web
	Biosearch Technologies
	IDT Technologies
	Operon
	Proligo
	Sigma-Genosys
	TriLink Biotechnologies

Varios:

	geNORM (Software de normalización de genes de referencia)
	RefSeq (Base de datos de secuencias de referencia)
	Spidey (cDNA to genomic alignment tool)
	Todo sobre los Molecular Beacons
	Links y herramientas generales de Biología Molecular
	TATAA Biocenter

Thermocycler calibration guide